Sequence and characteristics or IS900, an insertion element identified in a human Crohn's disease isolate orMycobacterium paratuberculosis
نویسندگان
چکیده
منابع مشابه
Specific detection of Mycobacterium paratuberculosis by DNA hybridisation with a fragment of the insertion element IS900.
This paper describes the evaluation of a newly developed DNA probe for Mycobacterium paratuberculosis. DNA probe PCR278 is a 278 bp fragment obtained by polymerase chain reaction (PCR) amplification of the 5'-region of IS900, an insertion element contained in the genome of M paratuberculosis. This DNA probe can specifically distinguish M paratuberculosis from a wide range of other organisms, in...
متن کاملCharacterization of ISApl1, an insertion element identified from Actinobacillus pleuropneumoniae field isolate in China.
An insertion sequence (IS), designated ISApl1, was identified in Actinobacillus pleuropneumoniae. It was 1072 bp in length, and contained a large open reading frame (ORF), which encoded a putative transposase whose sequence was similar to that of transposases of various IS elements of the IS30 family. Another small ORF, a putative antisense repressor of transposase, was located in the opposite ...
متن کاملComparison and Validation of IS900-Sequence andPutative Sequences as a Specific Target to DetectMycobacterium avium subsp. Paratuberculosis
1Department of Pathobiology, College of Veterinary Medicine, Nursing & Allied Health, Tuskegee University, Analysis, Tuskegee, Alabama, USA 2Pharmaceutical Sciences, Mercer University Atlanta Campus, Atlanta, Georgia, USA 3Thompson-Bishop-Sparks State Diagnostic Laboratory, Auburn, Alabama, USA 4Department of Animal Sciences, Auburn University, Auburn, Alabama, USA 5Center for Computational Epi...
متن کاملDetection of Mycobacterium avium subspecies paratuberculosis infection in two different camel species by conventional and molecular techniques
Paratuberculosis (John’s disease) is infectious and chronically progressive granulomatous disease which affects domestic and wild ruminants. The causative agent is Mycobacterium avium paratuberculosis (MAP), a slow growing mycobactin dependent acid-fast bacillus. We investigated the detection and frequency of MAP in apparently healthy dromedary and Bactrian camels by insertion...
متن کاملRecent insertion of an Alu element within a polymorphic human-specific Alu insertion.
Alu elements are a family of short interspersed repeats that have mobilized throughout primate genomes by retrotransposition over the past 65 Myr of primate evolution (for a review, see Deininger and Batzer 1993). In the human genome, Alu elements exist in copy numbers of approximately 500,000 per haploid genome, representing approximately 5% of the genome, and they may be classified into group...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.22.9063